Laboratory of Protein Engineering
Responsible person: prof. RNDr. Erik Sedlák, DrSc.
Mgr. Mária Tomková, PhD.
Analyses
Scientific
The Protein Engineering Laboratory focuses on the development and optimization of proteins and binding molecules using directed evolution approaches, particularly ribosome display technology. Experimental workflows include the preparation of genetic variant libraries, PCR amplification, gene cloning, and electrophoretic DNA analysis, together with verification of genetic constructs. This cell-free platform enables the selection of protein variants without the need for cellular transformation, significantly expanding library diversity and accelerating the identification of candidate molecules for downstream analysis and applications.
Commercial
We provide comprehensive molecular biology and protein engineering services designed to accelerate the development of binding molecules — from genetic construct design, PCR analysis, and gene cloning to electrophoretic DNA quality control. By leveraging ribosome display technology, we efficiently identify and optimize promising molecular candidates, substantially shortening the path from experimental concept to functional solution.
Services
Protein Engineering – Ribosome Display
- Design and preparation of plasmid vectors and genetic constructs
- Preparation and diversification of genetic variant libraries
- Selection of binding molecules using ribosome display
- Iterative optimization of affinity and specificity of protein variants
- Basic testing and verification of protein constructs
Molecular Biology Services
- Conventional and gradient PCR amplification of DNA
- Gene and genetic construct cloning
- Agarose gel electrophoresis and DNA fragment analysis
- Sequencing-based verification of genetic constructs
Laboratory of Protein Engineering
The laboratory is equipped with key instrumentation for molecular biology experiments, including a PCR thermocycler (Eppendorf Mastercycler X50S) and a PCR workstation (Biosan). It features systems for agarose gel electrophoresis (Bio-Rad PowerPac HC), a UV transilluminator (Herolab) for DNA visualization, a refrigerated centrifuge (Eppendorf 5424 R), and thermomixers (Eppendorf, Biosan), enabling efficient sample separation and controlled incubation of prepared samples.
Laboratory of Biotechnology and Cell Physiology
The laboratory employs cellular models and advanced analytical techniques to investigate the expression, localization, and biological activity of recombinant proteins. Using confocal microscopy, flow cytometry, spectroscopic methods, and metabolic analyses, we evaluate protein–cell interactions, stability, cytotoxicity, and functional properties within biological systems.
Laboratory of Laser Spectroscopy
The Laboratory of Laser Spectroscopy focuses on the investigation and detailed analysis of molecular processes using advanced spectroscopic and optical techniques with high sensitivity and temporal resolution.
Laboratory of Biothermo-dynamics and Optical Spectroscopy
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Laboratory of Molecular Biology
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Laboratory of Cell Cultures
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Laboratory of Time Resolved Microscopy
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Laboratory of Raman spectroscopy
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Synthesis of organic molecules
Synthesis of organic molecules for biochemical and cellular assays.