Laboratory of Time Resolved Microscopy
Analyses
Scientific
The laboratory focuses on advanced studies of fluorescence and phosphorescence processes in both macro- and microscale systems using state-of-the-art time-resolved imaging and spectroscopic technologies. The facility enables detailed analysis of excited-state dynamics, fluorescence lifetimes, energy transfer mechanisms, and biomolecular interactions in biological, chemical, and material systems. The integration of microscopic and spectroscopic approaches allows the investigation of processes at both the single-cell level and within complex biological systems.
Commercial
Through its specialized instrumentation and expertise in fluorescence-based techniques, the facility provides support for fundamental research, biomedical and biotechnological applications, the development of diagnostic methods, the testing of novel materials, and collaborative projects with academic and industrial partners.
Laboratory of Protein Engineering
The laboratory is equipped with key instrumentation for molecular biology experiments, including a PCR thermocycler (Eppendorf Mastercycler X50S) and a PCR workstation (Biosan). It features systems for agarose gel electrophoresis (Bio-Rad PowerPac HC), a UV transilluminator (Herolab) for DNA visualization, a refrigerated centrifuge (Eppendorf 5424 R), and thermomixers (Eppendorf, Biosan), enabling efficient sample separation and controlled incubation of prepared samples.
Laboratory of Biotechnology and Cell Physiology
The laboratory employs cellular models and advanced analytical techniques to investigate the expression, localization, and biological activity of recombinant proteins. Using confocal microscopy, flow cytometry, spectroscopic methods, and metabolic analyses, we evaluate protein–cell interactions, stability, cytotoxicity, and functional properties within biological systems.
Laboratory of Laser Spectroscopy
The Laboratory of Laser Spectroscopy focuses on the investigation and detailed analysis of molecular processes using advanced spectroscopic and optical techniques with high sensitivity and temporal resolution.
Laboratory of Biothermo-dynamics and Optical Spectroscopy
The Laboratory of Biothermodynamics and Optical Spectroscopy focuses on the biophysical characterization of biomacromolecules, the investigation of ligand interactions with proteins and nucleic acids, and the analysis of the stability and functional properties of biological complexes using advanced optical and spectroscopic techniques.
Laboratory of Molecular Biology
The laboratory specializes in the comprehensive processing of biological samples, with a particular focus on proteins and nucleic acids. Its expertise encompasses the isolation, purification, targeted molecular modification, and detailed structural and functional characterization of these biomolecules.
Laboratory of Cell Cultures
The laboratory provides the preparation of both live and fixed cell samples and employs advanced cell biology, imaging, and bioanalytical methodologies for the assessment of cell viability, proliferation, cellular responses to biologically active compounds, and the analysis of molecular and physiological changes at the single-cell level.
Laboratory of Confocal Microscopy
The laboratory utilizes a state-of-the-art confocal microscope for high-resolution 2D and 3D fluorescence imaging of cells, tissues, and biomaterials, enabling the analysis of intracellular processes, biomolecular localization, the spatial organization of cellular structures, and dynamic changes induced by experimental conditions or biologically active compounds.
Laboratory of Flow Cytometry
The laboratory is equipped with a CytoFLEX SRT cell sorter (Beckman Coulter) for precise cell sorting and a CytoFLEX S analyzer (Beckman Coulter) for flow cytometric analysis. The instrumentation is complemented by a Class II laminar flow cabinet (ESCO) for the safe handling of biological materials, a refrigerated centrifuge (Eppendorf 5430 R), a Gene Pulser Xcell electroporation system (Bio-Rad) for cell transformation, and a QIAGEN TissueLyser III homogenizer.
Laboratory of Time Resolved Microscopy
The laboratory employs highly sensitive time-resolved measurement techniques for the characterization of cellular processes, molecular interactions, and changes induced by external stimuli or biologically active compounds, providing insights that are not accessible through conventional imaging methods.
Laboratory of Raman spectroscopy
The laboratory enables non-invasive and contact-free identification of molecular composition, structural changes, and chemical interactions within analyzed systems, providing detailed information on biomolecules, cellular structures, nanomaterials, and functional surfaces.
Laboratory of Recombinant Proteins
We perform protein purification using affinity chromatography and size-exclusion chromatography, with purity assessment carried out through SDS-PAGE and HPLC systems. For biophysical characterization, we employ differential scanning calorimetry (MicroCal VP-Capillary DSC) and circular dichroism spectroscopy (Chirascan V100), enabling detailed analysis of protein stability, conformation, and molecular interactions. Our capabilities also include protein crystallization using the Crystal Gryphon robotic system and stereoscopic microscopy (Leica Microsystems Ivesta 3) for the optimization of crystallization conditions and subsequent crystal analysis.
Synthesis of organic molecules
Synthesis of organic molecules for biochemical and cellular assays.