CIB researchers mastering FLIM to target breast cancer
CIB researchers mastering FLIM to target breast cancer
The team of RNDr. Veronika Huntošová, PhD. has the opportunity to strengthen the cooperation with the group of Dr. Gyorgy Vámosi at the Institute of Biophysics and Cell Biology of the University of Debrecen in Hungary. During 20-31. July 2025, CIB researchers performed fluorescence live imaging of breast cancer labelled with fluorescent proteins developed using protein engineering in the CIB laboratories.
Dr. Vámosi's group and Dr. Huntošová's team at the facility in Debrecen.
The changes in the endomembrane system of breast cancer cells were monitored after treatment by DARPin proteins that specifically bind to HER2 receptors of these cells. These receptors are among the key factors for the invasiveness of the cells. The experiments were carried out as part of the Breast cancer organoid on a chip for fluorescence lifetime imaging of autophagy and apoptosis induced by targeted treatment with designed ankyrin repeat protein project. The project is supported by EU NextGenerationEU through the Recovery and Resilience Plan for Slovakia under project number 09I03-03-V04-00007.
PhD students T. Gulyásová and A. Benziane perform fluorescence lifetime measurements of DARPin-FITC in cancer cells at the Debrecen facility.
The access to imaging technologies and services via the Cellular Imaging Hungary Node (University of Debrecen, Hungary) as a part of Euro-BioImaging (https://www.eurobioimaging.eu) was supported with canSERV project funded under the Horizon Europe programme under grant agreement number 101058620. canSERV is providing cutting-edge cancer research services across Europe.
PhD students T. Gulyásová and N. Bilakovics carry out FLIM measurements at the facility in Debrecen to identify changes in the endomembrane system of cancer cells.
