Mgr. Ľuboš Ambro, PhD.
Current positions:
- Senior scientist, Center of Interdisciplinary Bioscienes, Technology and Innovation Park, Pavol Jozef Šafárik University, Košice
Past positions:
- 2014 – 2021: Researcher – Institute of Experimental Medicine, Faculty of Medicine, PJŠU
Education:
- 2009 – 2014: PhD study, Faculty of Natural Sciences – Comenius University, Bratislava (Research conducted at Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava)
- 2007 – 2009: Master’s study, Faculty of Natural Sciences – Comenius University, Bratislava (Research conducted at Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava)
- 2004 – 2007: Bachelor’s study, Faculty of Natural Sciences – Comenius University, Bratislava
Research interests:
- Recombinant protein production in bacterial and insect cell systems (E. coli, Sf9)
- Membrane proteins, nanodiscs and extracellular vesicles
- Surface and secretome proteomics of bacteria and eukaryotic cells
- Protein–cofactor interactions (flavoproteins, biliverdin-binding proteins)
Languages:
- English, Slovak
Research training:
- FEBS practical course: Advanced proteomics. Brixen, Italy, 27.7. – 3.8.2019
- EMBO practical course: Breathless microbes. Wageningen, Netherlands, 24. – 28.7.2017
- EMBO practical course: High-throughput Protein Production and Crystallization. Harwell, United Kingdom, 15. – 25.5.2013
- FEBS practical course: Advanced Methods in Macromolecular Crystallization V. Nové hrady, Czech Republic, 22. – 29.6.2012
Technical Expertise:
- Microbiology: aerobic and anaerobic cultivation of bacteria
- Molecular biology: DNA/RNA isolation, PCR, cloning (restriction-based, Gibson, SLIC)
- Protein bioinformatics: structural modelling, visualization and stability prediction
- Recombinant protein expression: E. coli and Spodoptera frugiperda systems
- Protein purification: affinity chromatography (IMAC, protein A/G, heparin), ion exchange, size exclusion chromatography
- Biophysical characterization: DSC, UV-Vis spectroscopy, enzyme kinetics
- Proteomics sample preparation (secretome and surface shaving approaches)
Teaching:
- Protein Engineering (MSc, Biophysics, Faculty of Science, PJŠU)
- Experimental Methods in Biophysics (MSc, Biophysics, Faculty of Science, PJŠU)
- Genetic Manipulations (BSc, Biology, Faculty of Science, PJŠU)
Diploma students:
- Supervised 8 defended diploma students (Faculty of Medicine and Faculty of Science, PJŠU)
Publications:
- 20 publications in international peer-reviewed journals (WOS and Scopus databases), cited more than 200 times, Hirsch index (Scopus) 9
